作者
Tze Ping Loh, Chung Shun Ho, Michaela F Hartmann, Rosita Zakaria, Clara Wai Shan Lo, Sjoerd van den Berg, Yolanda B de Rijke, Brian R Cooke, Kirsten Hoad, Peter Graham, Stephen R Davies, Lindsey G Mackay, Stefan A Wudy, Ronda F Greaves
发表日期
2020/9/25
期刊
Clinical Chemistry and Laboratory Medicine (CCLM)
卷号
58
期号
10
页码范围
1731-1739
出版商
De Gruyter
简介
Objectives
Our recent survey of 44 mass spectrometry laboratories across 17 countries identified variation in internal standard (IS) choice for the measurement of serum/plasma 17α-hydroxyprogesterone (17OHP) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The choice of IS may contribute to inter-method variations. This study evaluated the effect of two common isotopically labeled IS on the quantification of 17OHP by LC-MS/MS.
Methods
Three collaborating LC-MS/MS laboratories from Asia, Europe and Australia, who routinely measure serum 17OHP, compared two IS, (1) IsoSciences carbon-13 labeled 17OHP-[2,3,4-13C3], and (2) IsoSciences deuterated 17OHP-[2,2,4,6,6,21,21,21-2H]. This was performed as part of their routine patient runs using their respective laboratory standard operating procedure.
Results
The three laboratories measured 99, 89, 95 independent samples …
学术搜索中的文章
TP Loh, CS Ho, MF Hartmann, R Zakaria, CWS Lo… - Clinical Chemistry and Laboratory Medicine (CCLM), 2020
