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Idiopathic Pulmonary Fibrosis (IPF) is a progressive lung disease characterized by abnormal tissue fibrosis. It affects 30,000 to 40,000 individuals every year in the United States, mainly older adults, with no known cure. Two antifibrotic drugs, Nintedanib and Pirfenidone, have been shown to slow the progression of IPF but only a small portion of patients respond to these therapies, with no proven ability to reduce fatalities. Lung tissue from 16 IPF donors and 27 aged-matched controls were subjected to a solvent-based extraction method that partitions polar lipids, metabolites, and proteins in a multiphase process. Lipids were analyzed by liquid chromatography coupled mass-spectrometry (LC-MS/MS) in positive and negative mode to maximize the lipid coverage. Metabolites were analyzed by gas-chromatography coupled with mass spectrometry. The proteins were trypsinized into peptides …