作者
Jarone Pinhassi, Ulla Li Zweifel, Aî Hagstroëm
发表日期
1997/9
期刊
Applied and Environmental Microbiology
卷号
63
期号
9
页码范围
3359-3366
简介
The density of specific aquatic bacteria was determined by use of whole-genome DNA hybridization towards community DNA. From a coastal marine environment (northern Baltic Sea), 48 specific bacteria were isolated on solid media over a 1-year period. Based on the presented hybridization protocol, the total density of the isolates ranged between 7 and 69% of the bacteria determined by acridine orange direct counts. When compared to the number of nucleoid-containing cells, the range increased to 29 to 111%. Thus, our results showed that bacteria able to form colonies on solid media accounted for a large fraction of the bacterioplankton. There were significant changes in the density of the different bacteria over the year, suggesting that bacterioplankton exhibit a seasonal succession analogous to phytoplankton. The bacteria studied were of diverse phylogenetic origin, being distributed among the alpha, beta …
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J Pinhassi, UL Zweifel, A Hagstroëm - Applied and Environmental Microbiology, 1997