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Plants have evolved several metabolic pathways as a response to environmental stress like low temperatures. In this perspective, it is paramount to highlight physiological mechanisms of plant responses to changing environments. To determine the impact of cold stress on the physiological attributes of high altitude plant natives, i.e. Pedicularis punctata and Plantago major, we studied the protein, sugar and proline contents, as well as abscisic acid (ABA) and indoleacetic acid (IAA) in leaves collected from three different altitudinal ranges in Himalayan region of Pakistan. Leaves were collected at the initial blooming phase from each altitudinal range i.e. 2400–2500 m, 2950–3250 m and 3500–3550 m. Temperature decreases with an increase in the altitude which induces cold acclimation. Both plant species showed significant variation in the concentrations of the ecophysiological attributes evaluated at the different …