作者
Frank Ulrich Weiss, Ines J Marques, Joost M Woltering, Danielle H Vlecken, Ali Aghdassi, Lars Ivo Partecke, Claus–Dieter Heidecke, Markus M Lerch, Christoph P Bagowski
发表日期
2009/12/1
期刊
Gastroenterology
卷号
137
期号
6
页码范围
2136-2145. e7
出版商
WB Saunders
简介
BACKGROUND & AIMS
The infiltrating ductal adenocarcinoma of the pancreas is among the most lethal of all solid malignancies, largely owing to a high frequency of early metastasis. We identified microRNA-10a (miR-10a) as an important mediator of metastasis formation in pancreatic tumor cells and investigated the upstream and downstream regulatory mechanisms of miR-10a.
METHODS
Northern blot analysis revealed increased expression levels of miR-10a in metastatic pancreatic adenocarcinoma. The role of miR-10a was analyzed by Morpholino and short interfering RNA transfection of pancreatic carcinoma cell lines and resected specimens of human pancreatic carcinoma. Metastatic behavior of primary pancreatic tumors and cancer cell lines was tested in xenotransplantation experiments in zebrafish embryos.
RESULTS
We show that miR-10a expression promotes metastatic behavior of pancreatic …
引用总数
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