作者
Wémeson F da Silva, Manuel J Simões, Robson C Gutierre, Mizue I Egami, Antenor A Santos, Marta M Antoniazzi, Gisela R Sasso, Maria José T Ranzani-Paiva
发表日期
2017/1/1
期刊
Fish & Shellfish Immunology
卷号
60
页码范围
502-508
出版商
Academic Press
简介
Intestine mast cells/eosinophilic granule cells (MCs/EGC) of the marine species Centropomus parallelus (fat snook) were first studied using light and electron microscopy techniques. Mast cells are cells from the connective tissue found in almost all organs and tissues of vertebrates. In fish, they appear in greater numbers in parts of their bodies that are exposed to their environment, such as skin, gills and intestine. The granules in fat snook's mast cell contain a variety of substances, such as histamine, heparin, chondroitin sulfate, serotonin, proteases and cytokines. The present study of intestine MCs/EGC was carried out in 20 specimens of fat snook. Samples of tissue were fixed in Bouin solution and in buffered formalin. Ferric hematoxylin - Congo red, pH6 acridine orange, pH2.5 and pH0,5 Alcian Blue (AB), toluidine blue, PAS, AB + PAS and immunohistochemistry protocols were used. In the mucosa and …
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WF da Silva, MJ Simões, RC Gutierre, MI Egami… - Fish & Shellfish Immunology, 2017