作者
Wémeson F da Silva, Mizue I Egami, Antenor A Santos, Marta M Antoniazzi, Marcelo Silva, Robson C Gutierre, Maria José R Paiva
发表日期
2011/10/1
期刊
Fish & shellfish immunology
卷号
31
期号
4
页码范围
571-577
出版商
Academic Press
简介
The cytochemical, immunocytochemical and ultrastructural characteristics of leukocytes and thrombocytes in the peripheral blood of the fat snook (Centropomus paralellus) – a fish occurring in Brazil – were investigated. The cytochemical methods were performed to demonstrate four enzymatic reactions – o-toluidine-hydrogen peroxide, naphtol AS-MX phosphate, naphtol AS-BI phosphate and alpha-naphtil acetate to detect myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP) and non-specific esterase (α-NAE), respectively – and two non-enzymatic ones – Periodic-Acid Schiff (PAS) and Sudan black B (SBB) to detect the occurrence of glycogen and phospholipids, respectively. Immunocytochemical method utilizing polyclonal rabbit antibody against mammal metalloproteinases (MMPs) 2 and 9 were done. Standard method for Electron Microscopy (EM) was applied for the ultrastructural …
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