作者
Tomoko Nakamoto, Xinlong Wang, Naoki Kawazoe, Guoping Chen
发表日期
2014/10/1
期刊
Colloids and Surfaces B: Biointerfaces
卷号
122
页码范围
316-323
出版商
Elsevier
简介
In recent years, various approaches have been taken to generate functional muscle tissue by tissue engineering. However, in vitro methods to generate smooth muscle with physiologically aligned structure remains limited. In order to mimic the in vivo highly organized structure of smooth muscle cells, we used micropatterning technology for engineering parallel aligned cells. In this study, a gradient micropattern of different width of cell-adhesive polystyrene stripes (5, 10, 20, 40, 60, 80, 100, 200, 400, 600, 800 and 1000 μm) was prepared and the effects of micropattern width on human mesenchymal stem cells (hMSCs) orientation, morphology and smooth muscle cell differentiation were investigated. The width of micropattern stripes showed obvious effect on cell orientation, morphology and smooth muscle cell differentiation. The cells showed higher degree of orientation when the micropattern stripes became …
学术搜索中的文章
T Nakamoto, X Wang, N Kawazoe, G Chen - Colloids and Surfaces B: Biointerfaces, 2014