查看文章

The β-chemokine macrophage inflammatory protein-1α (MIP-1α) is chemotactic for many hemopoietic cell types and can inhibit hemopoietic stem cell (HSC) proliferation, effects mediated through G-protein coupled heptahelical receptors. We have isolated cDNAs for seven chemokine receptors, CCR-1 to -5, MIP-1αRL1, and a novel cDNA, D6. Chinese hamster ovary cells expressing CCR-1, -3, -5, and D6 bound ¹²⁵I-murine MIP-1α: the order of affinity was D6 > CCR-5 > CCR-1 > CCR-3. Each bound a distinct subset of other β-chemokines: the order of competition for ¹²⁵I-murine MIP-1α on D6 was murine MIP-1α > human and murine MIP-1β > human RANTES∼JE > human MCP-3 > human MCP-1. Human MIP-1α and the α-chemokines did not compete. Like other chemokine receptors, D6 induced transient increases in [Ca²⁺] in HEK 293 cells upon ligand binding. D6 mRNA was abundant in lung and …