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The Notch signaling pathway has been shown to play a role in bone marrow−derived stromal cell differentiation, however, the precise outcome of Notch activation remains controversial. The aim of this study was to evaluate the effect of Notch signaling in primary human bone marrow−derived stromal cells (hBMSCs).

hBMSCs were transduced to >90% with lentiviral vectors containing either human notch1 intracellular domain (NICD), jagged1, or dominant negative mastermind1. Cells were exposed to adipogenic and osteogenic differentiation stimuli and differentiation was quantified by oil red or alizarin red staining, alkaline phosphatase liver/bone/kidney (ALPL) activity and expression of adipogenic or osteogenic marker genes.

NICD and jagged1 transgene−expressing hBMSCs demonstrated enhanced mineralization, nodule formation, and ALPL activity in …