作者
Laura Pierdomenico, Laura Bonsi, Mario Calvitti, Damiano Rondelli, Mario Arpinati, Gabriella Chirumbolo, Ennio Becchetti, Cosetta Marchionni, Francesco Alviano, Valentina Fossati, Nicola Staffolani, Michele Franchina, Alberto Grossi, Gian Paolo Bagnara
发表日期
2005/9/27
期刊
Transplantation
卷号
80
期号
6
页码范围
836-842
出版商
LWW
简介
Background.
Bone marrow mesenchymal stem cells (MSCs) are currently being investigated in preclinical and clinical settings because of their multipotent differentiative capacity or, alternatively, their immunosuppressive function. The aim of this study was to evaluate dental pulp (DP) as a potential source of MSCs instead of bone marrow (BM).
Methods.
Flow cytometric analysis showed that DP-MSCs and BM-MSCs were equally SH2, SH3, SH4, CD29 and CD 166 positive. The in vitro proliferative kinetics of MSCs were measured by 3H-thymidine incorporation uptake. The immunosuppressive function of MSCs was then tested by coculturing PHA-stimulated allogeneic T cells with or without MSCs for 3 days.
Results.
BM-MSCs could be differentiated in vitro into osteogenic, chondrogenic and adipogenic lineages. DP-MSCs showed osteogenic and adipocytic differentiation, but did not differentiate into chondrocytes …
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L Pierdomenico, L Bonsi, M Calvitti, D Rondelli… - Transplantation, 2005