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The Depression Anxiety Stress Scales-21 (DASS-21) is a commonly used scale to briefly assess psychological distress in both research and clinical practice. Although the DASS-21 has been validated for use in numerous cultural, racial, and cisgender populations, its invariance for transgender and nonbinary (TGNB) populations has yet to be tested. Considering prior findings of invariance for similar measures of depression and anxiety with TGNB samples, it is important to understand whether scores for the DASS-21 differ as a function of gender identity. Using a sample of 546 (52.5%) cisgender sexual minority and 494 (47.5%) TGNB participants (N= 1040), configural, metric, and scalar invariance were tested through multiple group confirmatory factor analysis (CFA) for both cisgender versus broad TGNB participants as well as within TGNB identity groups. The DASS-21 demonstrated all levels of invariance for …