作者
MASATOSHI TANAKA, KOICHI TAKAHASHI, TAKESHI SAIKA, INTETSU KOBAYASHI, TAKAMASA UENO, JOICHI KUMAZAWA
发表日期
1998/6
期刊
The Journal of urology
卷号
159
期号
6
页码范围
2215-2219
出版商
Wolters Kluwer
简介
Purpose
To investigate the development of fluoroquinolone resistance among Neisseria gonorrhoeae isolates in Japan and the frequency and patterns of mutations involving the GyrA and ParC proteins, which confer quinolone resistance to the bacteria, in isolates.
Materials and Methods
Antimicrobial susceptibilities of 145 gonococcal isolates, including 79 isolated from February 1992 through February 1993 and 66 isolated from February 1995 through February 1996, to six fluoroquinolones and several other antibiotics were compared with those of 27 isolates obtained from 1981 through 1984. To identify mutations in gyrA and parC genes of the isolates, the quinolone resistance-determining regions of the gyrA and parC genes were PCR-amplified and the PCR products were directly sequenced.
Results
The minimum inhibitory concentration for 90% of strains (MIC90) values of norfloxacin for the isolates from 1992 …
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