作者
Mei Ming, Baozhong Zhao, Christopher R Shea, Palak Shah, Lei Qiang, Steven R White, Diane M Sims, Yu-Ying He
发表日期
2015/4/1
期刊
Journal of Allergy and Clinical Immunology
卷号
135
期号
4
页码范围
936-945. e4
出版商
Mosby
简介
Background
Skin barrier integrity requires a highly coordinated molecular system involving the structural protein filaggrin (FLG). Mutational loss of the skin barrier protein FLG predisposes subjects to the development of atopic dermatitis (AD).
Objective
We sought to determine the role of sirtuin 1 (SIRT1) in skin barrier function, FLG expression, and development of AD.
Methods
Skin histology of mice with skin-specific SIRT1 deletion and wild-type control animals was examined by using hematoxylin and eosin staining. Protein and mRNA abundance was analyzed by means of immunoblotting, immunohistochemistry, immunofluorescence, and RT-PCR. Serum antibody levels were assessed by means of ELISA.
Results
Here we show that FLG is regulated by the protein deacetylase SIRT1 and that SIRT1 is critical for skin barrier integrity. Epidermis-specific SIRT1 ablation causes AD-like skin lesions in mice, and mice with …
学术搜索中的文章
M Ming, B Zhao, CR Shea, P Shah, L Qiang, SR White… - Journal of Allergy and Clinical Immunology, 2015