作者
Maojun Wang, Pengcheng Wang, Fan Liang, Zhengxiu Ye, Jianying Li, Chao Shen, Liuling Pei, Feng Wang, Jiang Hu, Lili Tu, Keith Lindsey, Daohua He, Xianlong Zhang
发表日期
2018/1
期刊
New Phytologist
卷号
217
期号
1
页码范围
163-178
简介
- Alternative splicing (AS) is a crucial regulatory mechanism in eukaryotes, which acts by greatly increasing transcriptome diversity. The extent and complexity of AS has been revealed in model plants using highthroughput nextgeneration sequencing. However, this technique is less effective in accurately identifying transcript isoforms in polyploid species because of the high sequence similarity between coexisting subgenomes.
- Here we characterize AS in the polyploid species cotton. Using Pacific Biosciences singlemolecule longread isoform sequencing (IsoSeq), we developed an integrated pipeline for IsoSeq transcriptome data analysis (https://github.com/Nextomics/pipeline-for-isoseq).
- We identified 176 849 fulllength transcript isoforms from 44 968 gene models and updated gene annotation. These data led us to identify 15 102 fibrespecific AS events and estimate that c. 51.4% of homoeologous …
学术搜索中的文章
M Wang, P Wang, F Liang, Z Ye, J Li, C Shen, L Pei… - New Phytologist, 2018