作者
Kotamballi N Chidambara Murthy, GK Jayaprakasha, Vinod Kumar, Keerti S Rathore, Bhimanagouda S Patil
发表日期
2011/3/23
期刊
Journal of agricultural and food chemistry
卷号
59
期号
6
页码范围
2314-2323
出版商
American Chemical Society
简介
The current study was an attempt to elucidate the mechanism of human colon cancer cell proliferation inhibition by limonin and limonin glucoside (LG) isolated from seeds of Citrus reticulata. The structures of purified compounds were confirmed by NMR and quantified using HPLC. These compounds of more than 95% purity were subjected to proliferation inhibition assay using human colon adenocarcinoma (SW480) cells. The IC50 value of 54.74 and 37.39 μM was observed for limonin and LG, respectively at 72 h. Following confirmation of proliferation inhibition, pattern of DNA fragmentation and activation of caspase-3 of the cells treated with limonoids suggest involvement of apoptosis. Furthermore, reduction in the transcription ratio of bcl2/bax and induction of cytochrome c release from mitochondria to cytosol with treatment of limonoids confirm the activation of intrinsic apoptosis pathway. The activity of Bax and …
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KN Chidambara Murthy, GK Jayaprakasha, V Kumar… - Journal of agricultural and food chemistry, 2011