作者
Norman Sachs, Angelos Papaspyropoulos, Domenique D Zomer‐van Ommen, Inha Heo, Lena Böttinger, Dymph Klay, Fleur Weeber, Guizela Huelsz‐Prince, Nino Iakobachvili, Gimano D Amatngalim, Joep de Ligt, Arne van Hoeck, Natalie Proost, Marco C Viveen, Anna Lyubimova, Luc Teeven, Sepideh Derakhshan, Jeroen Korving, Harry Begthel, Johanna F Dekkers, Kuldeep Kumawat, Emilio Ramos, Matthijs FM van Oosterhout, G Johan Offerhaus, Dominique J Wiener, Eduardo P Olimpio, Krijn K Dijkstra, Egbert F Smit, Maarten van der Linden, Sridevi Jaksani, Marieke van de Ven, Jos Jonkers, Anne C Rios, Emile E Voest, Coline HM van Moorsel, Cornelis K van der Ent, Edwin Cuppen, Alexander van Oudenaarden, Frank E Coenjaerts, Linde Meyaard, Louis J Bont, Peter J Peters, Sander J Tans, Jeroen S van Zon, Sylvia F Boj, Robert G Vries, Jeffrey M Beekman, Hans Clevers
发表日期
2019/2/15
期刊
The EMBO journal
卷号
38
期号
4
页码范围
e100300
简介
Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non‐structural viral NS2 protein, and preferentially …
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N Sachs, A Papaspyropoulos, DD Zomer‐van Ommen… - The EMBO journal, 2019