作者
Isabel Bento, Cristina Peixoto, Vjacheslav N Zaitsev, Peter F Lindley
发表日期
2007/2/1
期刊
Acta Crystallographica Section D: Biological Crystallography
卷号
63
期号
2
页码范围
240-248
出版商
International Union of Crystallography
简介
The three-dimensional molecular structure of human serum ceruloplasmin has been reinvestigated using X-ray synchrotron data collected at 100 K from a crystal frozen to liquid-nitrogen temperature. The resulting model, with an increase in resolution from 3.1 to 2.8 Å, gives an overall improvement of the molecular structure, in particular the side chains. In addition, it enables the clear definition of previously unidentified Ca2+-binding and Na+-binding sites. The Ca2+ cation is located in domain 1 in a configuration very similar to that found in the activated bovine factor Va. The Na+ sites appear to play a structural role in providing rigidity to the three protuberances on the top surface of the molecule. These features probably help to steer substrates towards the mononuclear copper sites prior to their oxidation and to restrict the size of the approaching substrate. The trinuclear copper centre appears to differ from the …
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I Bento, C Peixoto, VN Zaitsev, PF Lindley - Acta Crystallographica Section D: Biological …, 2007