作者
C Peixoto, MFQ Sousa, AC Silva, MJT Carrondo, PM Alves
发表日期
2007/1/10
期刊
Journal of Biotechnology
卷号
127
期号
3
页码范围
452-461
出版商
Elsevier
简介
Rotavirus like particles (RLPs) constitute a potential vaccine for the prevention of rotavirus disease, responsible for the death of more than half a million children each year. Increasing demands for pre-clinical trials material require the development of reproducible, scaleable and cost-effective purification strategies as alternatives to the traditional laboratory scale CsCl density gradient ultracentrifugation methods commonly used for the purification of these complex particles. Self-assembled virus like particles (VLPs) composed by VP2, VP6 and VP7 rotavirus proteins (VLPs 2/6/7) were produced in 5l scale using the insect cells/baculovirus expression system. A purification process using depth filtration, ultrafiltration and size exclusion chromatography as stepwise unit operations was developed. Removal of non-assembled rotavirus proteins, concurrently formed particles (RLP 2/6), particle aggregates and products of …
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C Peixoto, MFQ Sousa, AC Silva, MJT Carrondo… - Journal of Biotechnology, 2007