作者
Daiwen Yang, Joel R Tolman, Natalie K Goto, Lewis E Kay
发表日期
1998/8
期刊
Journal of biomolecular NMR
卷号
12
页码范围
325-332
出版商
Kluwer Academic Publishers
简介
A triple resonance pulse scheme is presented for recording 13Cα-1Hα one-bond dipolar couplings in 15N, 13C labeled proteins. HNCO correlation maps are generated where the carbonyl chemical shift is modulated by the 13Cα-1Hα coupling, with the two doublet components separated into individual data sets. The experiment makes use of recently described methodology whereby the protein of interest is dissolved in a dilute solution of bicelles which orient above a critical temperature, thus permitting measurement of significant couplings (Tjandra and Bax, 1997a). An application to the protein ubiquitin is described.
引用总数
学术搜索中的文章
D Yang, JR Tolman, NK Goto, LE Kay - Journal of biomolecular NMR, 1998