作者
Michael Lienemann, Michaela A TerAvest, Juha‐Pekka Pitkänen, Ingmar Stuns, Merja Penttilä, Caroline M Ajo‐Franklin, Jussi Jäntti
发表日期
2018/11
期刊
Microbial Biotechnology
卷号
11
期号
6
页码范围
1184-1194
简介
Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR‐1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well‐characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose‐binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose‐coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate …
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M Lienemann, MA TerAvest, JP Pitkänen, I Stuns… - Microbial Biotechnology, 2018