作者
Charles J Choi, Alysia R Belobraydich, Leo L Chan, Patrick C Mathias, Brian T Cunningham
发表日期
2010/10/1
期刊
Analytical biochemistry
卷号
405
期号
1
页码范围
1-10
出版商
Academic Press
简介
Using both experimental assays and fluid-dynamic finite element simulation models, we directly compared the achievable performance limits of four distinct assay configurations for label-free detection of an analyte from a test sample on a biosensor surface. The assay configurations studied in this work included a biosensor incorporated into the bottom surface of a microplate well and a microfluidic channel. For each configuration, we compared assay performance for the scenario in which the entire bottom surface of the fluid-handling vessel is coated with capture ligands with assay performance for the scenario in which the capture ligands are applied in the form of localized spots. As a model system, we used detection of the protein biomarker tumor necrosis factor-alpha (TNF-α) using immobilized TNF-α capture antibody. Results show that the microfluidic assay format dramatically reduces the time required to …
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CJ Choi, AR Belobraydich, LL Chan, PC Mathias… - Analytical biochemistry, 2010