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Information regarding the local liquid velocity in bubble columns is of great interest for research into its performance. Common optical methods fail in bubble flows that have large void fractions because of the different refraction indices of the liquid and gaseous phases. The new X-ray particle tracking velocimetry (XPTV) described here solves the problem by the use of X-rays instead of light. X-rays penetrate a gas/liquid flow in straight lines. XPTV enables us to measure the three-dimensional velocity of the liquid phase. This method was applied and validated in two bubble columns. The same method is also applicable to opaque liquids.