作者
Willem Staels, Yannick Verdonck, Yves Heremans, Gunter Leuckx, Sofie De Groef, Carlo Heirman, Eelco de Koning, Conny Gysemans, Kris Thielemans, Luc Baeyens, Harry Heimberg, Nico De Leu
发表日期
2018/8
期刊
Diabetologia
卷号
61
页码范围
1804-1810
出版商
Springer Berlin Heidelberg
简介
Aims/hypothesis
The initial avascular period following islet transplantation seriously compromises graft function and survival. Enhancing graft revascularisation to improve engraftment has been attempted through virus-based delivery of angiogenic triggers, but risks associated with viral vectors have hampered clinical translation. In vitro transcribed mRNA transfection circumvents these risks and may be used for improving islet engraftment.
Methods
Mouse and human pancreatic islet cells were transfected with mRNA encoding the angiogenic growth factor vascular endothelial growth factor A (VEGF-A) before transplantation under the kidney capsule in mice.
Results
At day 7 post transplantation, revascularisation of grafts transfected with Vegf-A (also known as Vegfa) mRNA was significantly higher compared with non …
学术搜索中的文章
W Staels, Y Verdonck, Y Heremans, G Leuckx… - Diabetologia, 2018