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Study was performed for the biosynthesis of silver nanoparticle using Azadirachta indica mediated leaf extract and silver nitrate solution. After exposing the silver ions to the aqueous neem leaf extract, the rapid reduction of silver ions led to the construction of Ag-NPs in solution. The synthesized Ag-NPs were characterized using UV–Vis spectroscopy, FT-IR Spectroscopy, Scanning electron Microscopy and Energy Dispersive X-ray Spectroscopy (EDX). FT-IR result showed the biomolecules responsible for the bio-reduction of silver ion to Ag-NPs. SEM analysis showed the formation of the spherically agglomerated synthesized silver nanoparticles with size range from 31.84 ​nm to 47.85 ​nm. EDX profile showed elemental composition and the homogeneity of the synthesized silver nanoparticles and strong signal of the Ag-NPs was obtained in combination with weak signal of Carbon and Oxygen. The antibacterial …