作者
Kyosuke Matsuzaki, Kazutoshi Fujita, Kentaro Jingushi, Atsunari Kawashima, Takeshi Ujike, Akira Nagahara, Yuko Ueda, Go Tanigawa, Iwao Yoshioka, Koji Ueda, Rikinari Hanayama, Motohide Uemura, Yasushi Miyagawa, Kazutake Tsujikawa, Norio Nonomura
发表日期
2017/4/4
期刊
Oncotarget
卷号
8
期号
15
页码范围
24668
出版商
Impact Journals, LLC
简介
Background
Extracellular vesicles are lipid bilayer vesicles containing protein, messengerRNA and microRNA. Cancer cell-derived extracellular vesicles may be diagnostic and therapeutic targets. We extracted extracellular vesicles from urine of urothelial carcinoma patients and the control group to identify cancer-specific microRNAs in urinary extracellular vesicles as new biomarkers.
Materials and methods
microRNA from urinary extracellular vesicles extracted from 6 urothelial carcinoma patients and 3 healthy volunteers was analyzed. We verified candidate microRNAs in an independent cohort of 60 urinary extracellular vesicles samples. To normalize the microRNA expression level in extracellular vesicles, we examined the following in extracellular vesicles: protein concentration, CD9 intensity, amounts of whole miRNAs, RNA U6B small nuclear expression and the creatinine concentration of original urine …
学术搜索中的文章
K Matsuzaki, K Fujita, K Jingushi, A Kawashima… - Oncotarget, 2017