作者
Aaron D Goldberg, Laura A Banaszynski, Kyung-Min Noh, Peter W Lewis, Simon J Elsaesser, Sonja Stadler, Scott Dewell, Martin Law, Xingyi Guo, Xuan Li, Duancheng Wen, Ariane Chapgier, Russell C DeKelver, Jeffrey C Miller, Ya-Li Lee, Elizabeth A Boydston, Michael C Holmes, Philip D Gregory, John M Greally, Shahin Rafii, Chingwen Yang, Peter J Scambler, David Garrick, Richard J Gibbons, Douglas R Higgs, Ileana M Cristea, Fyodor D Urnov, Deyou Zheng, C David Allis
发表日期
2010/3/5
期刊
Cell
卷号
140
期号
5
页码范围
678-691
出版商
Elsevier
简介
The incorporation of histone H3 variants has been implicated in the epigenetic memory of cellular state. Using genome editing with zinc-finger nucleases to tag endogenous H3.3, we report genome-wide profiles of H3 variants in mammalian embryonic stem cells and neuronal precursor cells. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. The H3.3 chaperone Hira is required for H3.3 enrichment at active and repressed genes. Strikingly, Hira is not essential for localization of H3.3 at telomeres and many transcription factor binding sites. Immunoaffinity purification and mass spectrometry reveal that the proteins Atrx and Daxx associate with H3.3 in a Hira-independent manner. Atrx is required for Hira-independent localization of H3.3 at telomeres and for the repression of telomeric RNA. Our data demonstrate that …
引用总数
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AD Goldberg, LA Banaszynski, KM Noh, PW Lewis… - Cell, 2010