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An efficient transformation system was developed for tomato by studying several factors that affect the rate of Agrobacterium mediated transformation of explant tissue. Cotyledons of tomato were co–cultivated with Agrobacterium tumefaciens harboring a binary vector with two neomycin phosphotransferase (NPT) II genes and a mutant aroA gene. Over 100 transgenic plants were regenerated and rooted on medium containing kanamycin and eighty percent of these plants exhibited both NPT II enzyme activity and produced the mutant aroA protein. Progeny of aroA positive plants were tolerant to glyphosate at concentrations of 0.84 kg active ingredient/ha. The tolerance phenotype segregated in a manner consistent with Mendelian inheritance.