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Figure 1: Results of our flow cytometry-based assay of BTK expression in a normal donor, XLA patient and the mother.(A) FACS dot plot of CD20 and CD19 gated for lymphocytes. B cells (CD19 and CD20) were markedly decreased in the blood of the patient while the mother possessed a substantial number of circulating B cells (B) Histograms gated on CD14+ monocytes expressing BTK. Blue shaded area indicates the control antibody. The defective BTK expression in monocytes was seen in XLA patient and a bimodal pattern of normal and abnormal BTK expression was also observed in his mother, known as an obligate carrier. The father follows a pattern as the normal donor (result not shown).