作者
Mirna Perusina Lanfranca, Yaqing Zhang, Alexander Girgis, Samantha Kasselman, Jenny Lazarus, Illona Kryczek, Lawrence Delrosario, Andrew Rhim, Lada Koneva, Maureen Sartor, Lei Sun, Christopher Halbrook, Hari Nathan, Jiaqi Shi, Howard C Crawford, Marina Pasca di Magliano, Weiping Zou, Timothy L Frankel
发表日期
2020/4/1
期刊
Gastroenterology
卷号
158
期号
5
页码范围
1417-1432. e11
出版商
WB Saunders
简介
Background & Aims
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy that invades surrounding structures and metastasizes rapidly. Although inflammation is associated with tumor formation and progression, little is known about the mechanisms of this connection. We investigate the effects of interleukin (IL) 22 in the development of pancreatic tumors in mice.
Methods
We performed studies with Pdx1-Cre;LSL-KrasG12D;Trp53+/–;Rosa26EYFP/+ (PKCY) mice, which develop pancreatic tumors, and PKCY mice with disruption of IL22 (PKCY Il22–/–mice). Pancreata were collected at different stages of tumor development and analyzed by immunohistochemistry, immunoblotting, real-time polymerase chain reaction, and flow cytometry. Some mice were given cerulean to induce pancreatitis. Pancreatic cancer cell lines (PD2560) were orthotopically injected into C57BL/6 mice or Il22–/–mice, and …
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MP Lanfranca, Y Zhang, A Girgis, S Kasselman… - Gastroenterology, 2020