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Conventional practices of siting all biomass preprocessing operations at the biorefinery is widely believed to be the most cost-effective solution for feedstock supply because of economies of scale. However, biomass preprocessing operations could be decentralized by moving the preprocessing operations to distributed biomass preprocessing centers, also known as “depots” located near biomass sources. This study presents a comparative case study with multiple biomass resources to analyze biorefinery feedstock supply logistics designs having distributed depots and a primary depot co-located with the biorefinery. A mixed-integer linear programming model was developed to simultaneously optimize feedstock sourcing decisions, and optimal preprocessing depot locations and size, utilizing biomass resources from agricultural residue, energy and municipal solid waste to meet carbohydrate specifications and …