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Bioactive glass 45S5 (BG) has been proposed as a biomaterial and extensively studied on account of its superior bioactive behavior. In this paper, we report our results on thin film deposition of BG on Ti6Al4V alloy using pulsed laser deposition (PLD) technique. A Nd: YAG laser (532 nm) based pulsed laser deposition system has been used for this work. Post deposition, surface morphology, and chemical composition of the obtained films on Ti-alloy surface have been examined using scanning electron microscopy (SEM) and energy dispersive x-ray analysis (EDX). In vitro bioactivity of these PLD coated Ti-alloy samples was evaluated by immersing the BG coated Ti-alloy samples in simulated body fluid (SBF) for 10 days. SBF immersed samples have been analyzed using SEM, EDX, and micro Raman spectroscopic techniques. Superior growth of bone like apatite ie hydroxyapatite (HAP) have been observed on …