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Biofortification is a novel approach which can lead to the development of micronutrient dense staple crops. However, the role of microorganisms in improving the nutritional status of plants has been less investigated. In our study, one bacterial (Providencia sp. PW5) and three cyanobacterial strains CW1, CW2 and CW3 (Anabaena sp., Calothrix sp. and Anabaena sp. respectively) were evaluated in a field experiment, for their role in improving the nutritional quality of wheat grains, in terms of protein content and important micronutrients (Fe, Cu, Zn and Mn). An enhancement of 18.6% in protein content was recorded with PW5 + N₆₀P₆₀K₆₀ as compared to fertilizer control (N₆₀P₆₀K₆₀). Comparative analysis with fertilizer control (N₆₀P₆₀K₆₀) revealed that inoculation of Providencia sp. PW5 + N₆₀P₆₀K₆₀ recorded statically at par values for Zn accumulation (41.73 mg kg⁻¹) and resulted in an increase of 105.3, 36.7 …