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In order to avoid invasive sampling in pediatric patients, we examined the concentrations of LH and FSH in paired serum and urine samples from 43 children (age 0-16 yr) who had no interfering disorders or medication. Highly sensitive time-resolved immunofluorometric assay (IFMA) kits were obtained from Pharmacia-Wallac, Turku, Finland (LH and FSH Delfia®). The intraassay variation was< 8%. The detection limit of the assay was 0.015 IU/L for LH and 0.018 IU/L for FSH. These sensitivity levels cover the whole prepubertal range of LH and FSH levels. Urinary gonadotropin levels were aimed to be corrected by the urinary density [concentration×(0, 02/density-1)] and urinary creatinine levels (concentraiion/creatinine). The correlation between serum and urine gonadotropin values was good (see table). Besides good correlation, also the absolute concentrations were close to each other, which is not the case for …