作者
Architecture et Fonction des Macromolécules Biologiques Vincentelli Renaud 4 Cambillau Christian 4, Berkeley Structural Genomics Center Kim Rosalind 5 Kim Sung-Hou 5, China Structural Genomics Consortium Rao Zihe 6 Shi Yunyu 7, Integrated Center for Structure and Function Innovation Terwilliger Thomas C 8 Kim Chang-Yub 8 Hung Li-Wei 8 Waldo Geoffrey S 8, Israel Structural Proteomics Center Peleg Yoav 9 Albeck Shira 9 Unger Tamar 9 Dym Orly 9 Prilusky Jaime 9 Sussman Joel L 9, Joint Center for Structural Genomics Stevens Ray C 10 Lesley Scott A 10 11 Wilson Ian A 10 11, Midwest Center for Structural Genomics Joachimiak Andrzej 12 Collart Frank 12 Dementieva Irina 12 Donnelly Mark I 12 Eschenfeldt William H 12 Kim Youngchang 12 Stols Lucy 12 Wu Ruying 12 Zhou Min 12, Oxford Protein Production Facility Stuart David I 20 23 Owens Raymond J 20 23 Daenke Susan 20 23, Protein Sample Production Facility, Max Delbrück Center for Molecular Medicine Schütz Anja 21 Heinemann Udo 21, RIKEN Structural Genomics/Proteomics Initiative Yokoyama Shigeyuki 22, SPINE2-Complexes Büssow Konrad 21 24 24 Gunsalus Kristin C 18 24 24
发表日期
2008/2
来源
Nature methods
卷号
5
期号
2
页码范围
135-146
出版商
Nature Publishing Group US
简介
NOTE: In the version of this Review initially published, an author (B. Martin Hallberg) was left off of the author list. This information has been added to the HTML and PDF versions of the Review.
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
引用总数
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