作者
Yasmin Ahmad, Dhananjay Shukla, Iti Garg, Narendra K Sharma, Saurabh Saxena, VK Malhotra, Kalpana Bhargava
发表日期
2011/9
期刊
Functional & integrative genomics
卷号
11
页码范围
407-417
出版商
Springer-Verlag
简介
We have investigated the plasma proteome using 2D gel electrophoresis and matrix-assisted laser desorption/ionization tandem time of flight from patients with high altitude pulmonary edema (HAPE). A complete proteomic analysis was performed on 20 patients with HAPE and ten healthy sea level controls. In total, we have identified 25 protein spots in human plasma and found that 14 of them showed altered changes in HAPE patients, which mainly were acute phase proteins (APPs), compliment components, and apolipoproteins among others. Among the APPs, haptoglobin α2 chain, haptoglobin β chain, transthyretin, and plasma retinol binding precursor showed overexpression in HAPE patients as compared to controls. To validate the result of proteomic analysis, two proteins were selected for enzyme-linked immunosorbent assay and Western blotting analysis. Our data conclusively shows that two …
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Y Ahmad, D Shukla, I Garg, NK Sharma, S Saxena… - Functional & integrative genomics, 2011