作者
Elisabete Paula Barros, Nathalie Moreira, Giuliano Elias Pereira, Selma Gomes Ferreira Leite, Claudia Moraes Rezende, Paula Guedes de Pinho
发表日期
2012/11/15
期刊
Talanta
卷号
101
页码范围
177-186
出版商
Elsevier
简介
An automated headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed in order to quantify a large number of volatile compounds in wines such as alcohols, ester, norisoprenoids and terpenes. The procedures were optimized for SPME fiber selection, pre-incubation temperature and time, extraction temperature and time, and salt addition. A central composite experimental design was used in the optimization of the extraction conditions. The volatile compounds showed optimal extraction using a DVB/CAR/PDMS fiber, incubation of 5ml of wine with 2g NaCl at 45°C during 5min, and subsequent extraction of 30min at the same temperature. The method allowed the identification of 64 volatile compounds. Afterwards, the method was validated successfully for the most significant compounds and was applied to study the volatile …
学术搜索中的文章
EP Barros, N Moreira, GE Pereira, SGF Leite… - Talanta, 2012