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Water soluble fraction of ethanol extract of fruits of yctanthes arbor-tristis Linn (NATEF) was pharmacologically validated for its anti-inflammatory properties in experimental animals using various models such as Carrageenan-induced paw oedema and Cotton pellet induced granuloma in rats for anti-inflammatory activity. Extract was given orally at two different dose levels (250 and 500 mg/kg) once daily for three consecutive days, while indomethacin was administered as positive control. Studies have shown that the fruit extract has activity to prevent inflammation in the rodents. The anti-inflammatory effect was dose dependent and found to be statistically significant as compared to the control.