作者
Magdalena M Julkowska, Fionn McLoughlin, CARLOS S GALVAN‐AMPUDIA, Johanna M Rankenberg, Dorota Kawa, Maria Klimecka, Michel A Haring, Teun Munnik, Edgar E Kooijman, Christa Testerink
发表日期
2015/3
期刊
Plant, cell & environment
卷号
38
期号
3
页码范围
614-624
简介
Phosphatidic acid (PA) is an important signalling lipid involved in various stress‐induced signalling cascades. Two SnRK2 protein kinases (SnRK2.4 and SnRK2.10), previously identified as PA‐binding proteins, are shown here to prefer binding to PA over other anionic phospholipids and to associate with cellular membranes in response to salt stress in Arabidopsis roots. A 42 amino acid sequence was identified as the primary PA‐binding domain (PABD) of SnRK2.4. Unlike the full‐length SnRK2.4, neither the PABD‐YFP fusion protein nor the SnRK2.10 re‐localized into punctate structures upon salt stress treatment, showing that additional domains of the SnRK2.4 protein are required for its re‐localization during salt stress. Within the PABD, five basic amino acids, conserved in class 1 SnRK2s, were found to be necessary for PA binding. Remarkably, plants overexpressing the PABD, but not a non‐PA‐binding …
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