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A novel copper oxide nanoparticles was synthesized using ginger (Zingiber officinale Rosc.) rhizome extract from copper complex containing Schiff base ligand (metal precursor) via calcination approach and applied for antibacterial activities against gram negative species. The structure of the nanoparticles has been examined by XRD, SEM, TEM and FT-IR spectroscopic techniques. X-ray diffraction reveals the size of nanoparticles. Antibacterial potential of the prepared nanoparticles were carried out by using gram negative E. Coli bacteria and shows good antibacterial potential.