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Physical parameters for DNA delivery into oil palm embryogenic calli using the biolistic device have been successfully established. Helium pressures, distance from rupture disc to macrocarrier, distance from macrocarrier to stopping plate, distance from stopping plate to target tissue, vacuum pressures, number of bombardments, particle types and sizes and the effect of calcium chloride and spermidine on microcarrier-DNA binding have been optimizied. Embryogénic calli were bombarded with gold or tungsten particles coated with pEmuGN plasmid containing β-glucuronidase gene (uidA) driven by an Emu promoter. A completely randomized design was used to bombard a total of 200 plates (40 parameters × 5 replications) which were then incubated in a growth chamber at 28 °C. Two days after bombardment, the tissues were stained with GUS assay buffer for 16–20 h at 37 °C and the blue spots were counted …