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Transgenic oil palm (Elaeis guineensis Jacq.) plantlets were regenerated after using Agrobacterium tumefaciens-mediated transformation of embryogenic calli derived from young leaves of oil palm. The calli were transformed with a Agrobacterium strain, LBA4404, harbouring the plasmid pUBA, which carries a selectable marker gene (bar) for resistance to the glufosinate-ammonium and is driven by a maize ubiquitin promoter. Modifications of the transformation method, treatment of the target tissues using acetosyringone, exposure to a plasmolysis medium and physical injury via biolistics were applied. The main reasons for such modifications were to activate the bacterial virulence system and to subsequently increase the transformation efficiency. Transgenic oil palm was selected and regenerated on a medium containing glufosinate-ammonium.