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Introduction: Non-coding RNAs, particularly small Cajal body-associated RNAs (scaRNAs), play an important role in alternative splicing and maturation of mRNAs through pseudouridylatory modification. Our small non-coding RNA sequencing analysis has identified that scaRNA20 plays a major role in cardiomyocyte differentiation. In this study, we aim to elucidate the role of scaRNA20 during cardiac differentiation using in vitro induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iCMC) subjected to 3% hypoxic conditions.

Hypothesis: Overexpression (OE) of scaRNA20 in iPSCs leads to enhanced cardiomyogenesis and increases the endurance properties of cardiomyocytes by maintaining contractility under hypoxic conditions.

Methods and results: We have used OE and knockdown (KD) assays to delineate the role of scaRNA20 during the process of iPSC to iCMC differentiation. The video recordings of …