作者
Ilya Balmages, Janis Liepins, Stivens Zolins, Dmitrijs Bliznuks, Ilze Lihacova, Alexey Lihachev
发表日期
2021/3/1
期刊
Biomedical Optics Express
卷号
12
期号
3
页码范围
1609-1620
出版商
Optica Publishing Group
简介
In this study, an optical contactless laser speckle imaging technique for the early identification of bacterial colony-forming units was tested. The aim of this work is to compare the laser speckle imaging method for the early assessment of microbial activity with standard visual inspection under white light illumination. In presented research, the growth of Vibrio natriegens bacterial colonies on the solid medium was observed and analyzed. Both – visual examination under white light illumination and laser speckle correlation analysis were performed. Based on various experiments and comparisons with the theoretical Gompertz model, colony radius growth curves were obtained. It was shown that the Gompertz model can be used to describe both types of analysis. A comparison of the two methods shows that laser speckle contrast imaging, combined with signal processing, can detect colony growth earlier than standard …
引用总数
20212022202320244373
学术搜索中的文章
I Balmages, J Liepins, S Zolins, D Bliznuks, I Lihacova… - Biomedical Optics Express, 2021