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This study examines ionospheric total electron content (TEC) perturbations from six International Global Navigation Satellite System stations (GNSS) including GUAT-Guatemala, SSIA-El Salvador, INEG-Mexico, MANA-Nicaragua, MDO1-United States of America, and BOGT- Colombia for several days around the occurrence of a major earthquake (M 7.4 and depth 20.0 km) in Mexico, June 23, 2020 at 10:29 Local Time (LT). The INEG station in the North-Northwest of the epicenter at a distance of about 936 km indicated a positive TEC anomaly on June 18, 2020, which can be possibly viewed as an earthquake precursor due to its occurrence during a quiet geomagnetic storm and inactive solar activity. Study findings reveal that other TEC perturbations may not be related to the earthquake, because they appeared during geomagnetic activities. Moreover, the atmospheric parameters have significant and synchronous …