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Fundamental research on paper pulp produced from oil palm (Elaeis guineensis) empty fruit bunch (EFB) fiber was carried out in this study to gain better understanding of the residual lignin's structures. Residual lignin was isolated from oil palm empty fruit bunch (EFB) kraft-anthraquinone (AQ) pulp and soda-anthraquinone (AQ) pulp. Currently, there are two methods used for the isolation of residual lignin: acidic hydrolysis in dioxane-water 82:18 (v/v) with 0.1M HCL under reflux (Gellerstedt G, 1994) and enzymatic hydrolysis by using commercial cellulolytic enzymes in an acetate buffer with pH 4.5 at 45°C under continuous shaking. For acidic hydrolysis isolated lignin, the residual lignin is free from carbohydrates and other impurities but it may cause some structural modifications towards the lignin structure. Whereas the residual lignin obtained from enzymatic hydrolysis contains some carbohydrates and proteins …