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Crude hemicellulase by novel mycoparasitic Trichoderma asperellum (GenBank accession nos. KU878976) was characterized and used in synergy with commercial cellulase to enhance the hydrolysis of Oil-palm-empty-fruit-bunches (OPEFB). Hemicellulase complex was cellulase-free, but was dominated by β-1,4-xylanase and a few accessory enzymes such as β-xylosidase, α-arabinofuranosidase, acetyl xylan esterase and β-glucosidase. Based on xylanase assay, optimum enzyme activity was shown at 50 °C and pH 5.3; while thermal and pH stability were respectively at 50 °C (1 h) and pH 4.8–10. Moreover, the crude hemicellulase from T. asperellum could withstand up to 50% selected hydrolytic and fermentation inhibitors. Supplement of hemicellulase crude to Celluclast 1.5 L at 1FPU:5U enhanced the hydrolysis of alkaline-treated OPEFB biomass achieving a 91.7% saccharification yield, higher than 77.9 …