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Oleaginous microbes are promising platform strains for the production of renewable diesel and fatty-acid derived chemicals given their innate capacity to produce high lipid yields from lignocellulose-derived sugars. Substantial efforts have been conducted to engineer model oleaginous yeasts primarily on model feedstocks, but to enable lipid production from biomass, judicious strain selection based on phenotypes beneficial for processing, performance on realistic feedstocks, and process integration aspects from sugars to fuels should be examined holistically. To that end, here we report the bench-scale production of diesel blendstock using a biological-catalytic hybrid process based on oleaginous yeast. This work includes flask screening of 31 oleaginous yeast strains, evaluated based on baseline lipid profiles and sugar consumption with corn stover hydrolysate. Three strains were down-selected for bioreactor …