作者
Debbie Montjean, Pierre De La Grange, David Gentien, Audrey Rapinat, Stéphanie Belloc, Paul Cohen-Bacrie, Yves Menezo, Moncef Benkhalifa
发表日期
2012/1
期刊
Journal of assisted reproduction and genetics
卷号
29
页码范围
3-10
出版商
Springer US
简介
Purpose
Investigate in what extent sperm transcriptome of infertile men is different from that of fertile individuals.
Methods
Semen samples were collected for determination of sperm parameters as well as for RNA isolation. Gene expression profile was investigated in spermatozoa of 8 infertile and 3 fertile men by microarray analysis using the Affymetrix Chip HG-U133 Plus 2.0.
Result(s)
We observed up to 33-fold reduction expression of genes involved in spermatogenesis and sperm motility. Furthermore, there is an important decrease in expression of genes involved in DNA repair as well as oxidative stress regulation. In this study, we also show a striking drop in expression of histone modification genes.
Conclusion(s)
We found that transcription profile in germ cells of men with …
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D Montjean, P De La Grange, D Gentien, A Rapinat… - Journal of assisted reproduction and genetics, 2012