作者
Arturo O Rinaldi, Manru Li, Elena Barletta, Paolo D'Avino, Duygu Yazici, Yagiz Pat, Siobhan Ward, Daniel Burla, Ge Tan, Nima Askary, Rasmus Larsson, Jeremy Bost, Huseyn Babayev, Raja Dhir, Nicolas Gaudenzio, Mubeccel Akdis, Kari Nadeau, Cezmi A Akdis, Yasutaka Mitamura
发表日期
2024/1
期刊
Allergy
卷号
79
期号
1
页码范围
128-141
简介
Background
Epithelial barrier impairment is associated with many skin and mucosal inflammatory disorders. Laundry detergents have been demonstrated to affect epithelial barrier function in vitro using air–liquid interface cultures of human epithelial cells.
Methods
Back skin of C57BL/6 mice was treated with two household laundry detergents at several dilutions. Barrier function was assessed by electric impedance spectroscopy (EIS) and transepidermal water loss (TEWL) measurements after the 4 h of treatments with detergents. RNA sequencing (RNA‐seq) and targeted multiplex proteomics analyses in skin biopsy samples were performed. The 6‐h treatment effect of laundry detergent and sodium dodecyl sulfate (SDS) was investigated on ex vivo human skin.
Results
Detergent‐treated skin showed a significant EIS reduction and TEWL increase compared to untreated skin, with a relatively higher sensitivity …
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AO Rinaldi, M Li, E Barletta, P D'Avino, D Yazici, Y Pat… - Allergy, 2024